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Hoechst 33342 molecular probes

NettetHoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in combination with 5-bromo-2'-deoxyuridine (BrdU) labeling to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content. Nettet4. nov. 2016 · The influence of ABC transporters on the fluorescence of probes in SP and MP cells. FACS plots and mean fluorescent intensity (MFI) of sorted C6 SP (Hoechst 33342-effluxing) and MP (Hoechst 33342-retaining) cells after staining with CellROX (a, b), MTG (c, d) and JC-1 (e-g) in the presence or absence of verapamil.Fluorescence of …

Full article: Single molecule localization microscopy of the ...

NettetThis bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has … Nettet23. sep. 2024 · Microscopy was performed on a Carl Zeiss Axiovert 200 Μ with a Plan-Apochromat 63×/1.4NA oil lens and wavelength filter sets for detection of Hoechst 33342 (450 nm), Alexa Fluor 488 (520 nm) and Alex Fluor 555 (568 nm) The images were acquired using the high sensitivity digital camera AxioCam HRc for multicolor … eprinter noreply trustist.com https://more-cycles.com

Hoechst 33342, 20 mM solution in water - 5 ml

Nettet15. mar. 2013 · Amplex Red cholesterol assay kit was from Molecular Probes (Eugene, OR). All other chemicals and solvents used were of the highest available purity. Water was purified through a Millipore ... DNA bound Hoechst 33342 was excited with 351 nm and fluorescence was collected using 450/65 nm bandpass filter. In order to ... NettetHoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from ... Molecular Probes, Inc. Further information on Molecular Probes products, including product bibliographies, is available from your local distributor or directly NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … driving after a knee operation

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Category:Hoechst 33342 Solution (20 mM) - Thermo Fisher Scientific

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Hoechst 33342 molecular probes

Full article: Single molecule localization microscopy of the ...

NettetAdd 10 μ lof Hoechst 33342 to each 1 m of the cell suspension and mix thoroughly. Incubate the cells at 37°C for 5-15 minutes. 4. Centrifuge the cells at 1,000 rpm for five minutes at 4°C and discard the supernatant. 5. Resuspend cells in 1000 µl of 1X buffer A. 6. Add 5 μ lof PI to each 1 m of cell suspension and mix thoroughly. NettetHoechst 33342 Binding. Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell …

Hoechst 33342 molecular probes

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NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed Invitrogen™ Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water Fisher Scientific NettetAfter removing MitoTracker Red CMXRos, cells were treated with 200 nM MitoTracker Green FM (Molecular Probes) and incubated at 37 °C for 30 min. After removing MitoTracker Green FM, cells were treated with Hoechst 33342 (Dojindo) and incubated at 37 °C for 30 min. The fluorescence intensity of MitoTracker Red CMXRos, ...

NettetDescription. Hoechst 33342 is perhaps the most ubiquitous nuclear fluorescence stain for live and fixed cells and tissue sections. The cell permeable stain binds preferentially to AT regions of DNA. It is excited by UV light and emits blue fluorescence at 460nm when bound to DNA. Ready-to-use liquid formulation of high-purity Hoechst 33342. NettetThis bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has …

NettetHöchst 33342 ist eine beliebte zelldurchlässige nukleäre Gegenfärbung mit einer blauen Fluoreszenz bei Anbindung an dsDNA. Dieses Färbemittel wird häufig zur … NettetThermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques.Features of Hoechst 33342 Fluorescent Stain:

NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570).

NettetHoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA (preferentially binds to A-T base pairs). This dye is very useful to label double-stranded DNA and thus to visualize nuclei. Hoechst 33342 can be excited at ~355 nm by a UV light source (eg, UV laser beam or a mercury arc-lamp). eprinted.com reviewsNettet(B) Co-staining of viscosity-sensitive probes (DSP-16 and DSP-18), Dil (a commercial plasma membrane probe) with Hoechst 33342 (a commercial nuclei probe) … driving after a local anaestheticNettetThe bidirectional permeation characteristics of rhodamine 123 and Hoechst 33342, fluorescence probes of the binding sites on P-glycoprotein (P-gp), across monolayers of MDCK cells transfected with the human MDR1 gene (MDCK-MDR1) were investigated. The ratios of the apparent permeability coefficients … eprinting 3.0 reproplan reproplan-shop.de