NettetHoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in combination with 5-bromo-2'-deoxyuridine (BrdU) labeling to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content. Nettet4. nov. 2016 · The influence of ABC transporters on the fluorescence of probes in SP and MP cells. FACS plots and mean fluorescent intensity (MFI) of sorted C6 SP (Hoechst 33342-effluxing) and MP (Hoechst 33342-retaining) cells after staining with CellROX (a, b), MTG (c, d) and JC-1 (e-g) in the presence or absence of verapamil.Fluorescence of …
Full article: Single molecule localization microscopy of the ...
NettetThis bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has … Nettet23. sep. 2024 · Microscopy was performed on a Carl Zeiss Axiovert 200 Μ with a Plan-Apochromat 63×/1.4NA oil lens and wavelength filter sets for detection of Hoechst 33342 (450 nm), Alexa Fluor 488 (520 nm) and Alex Fluor 555 (568 nm) The images were acquired using the high sensitivity digital camera AxioCam HRc for multicolor … eprinter noreply trustist.com
Hoechst 33342, 20 mM solution in water - 5 ml
Nettet15. mar. 2013 · Amplex Red cholesterol assay kit was from Molecular Probes (Eugene, OR). All other chemicals and solvents used were of the highest available purity. Water was purified through a Millipore ... DNA bound Hoechst 33342 was excited with 351 nm and fluorescence was collected using 450/65 nm bandpass filter. In order to ... NettetHoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from ... Molecular Probes, Inc. Further information on Molecular Probes products, including product bibliographies, is available from your local distributor or directly NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … driving after a knee operation