Flp-in cho

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August undefined, 2024

WebLike Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage. In FLP-FRT, FLP is analogous to cre and FRT to loxP – FLP recognizes the FRT sites, and catalyzes recombination between them. WebOct 25, 2024 · Flp-In-CHO:huOX40 cells as well as Flp-In-CHO:vector cells generated in-house (Pieris) were cultivated in Ham’s F12 medium (Gibco, #31765-027) with 10% FBS and 500 µg/ml hygromycin B. All mammalian cell lines were grown at 37°C and 5% CO 2. Goat anti-rabbit IgG-PE conjugate was purchased from Dianova/Jackson …

Establishment of Flp-InTM CHO cell lines with double expression …

WebA, assay for the evaluation of the interaction between cells and surface-bound collectins. Step 1: incubation of AF 488-conjugated bioparticles with collectins. Step 2: incubation of … WebThe Flp-In cell lines should be maintained in medium containing Zeocin. For more information about pFRT/lacZeo and pcDNA5/FRT/CAT, please refer to the Flp-In System … green carpet and pink walls

A double-Flp-in method for stable overexpression of two …

WebThe Flp-In-CHO Cell Line was created by transfecting CHO cells with pFRT/ lac Zeo2 and selecting for Zeocin™-resistant clones Flp-In-CHO cell lines work well with Flp-In vectors that express gene from CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™, and pSecTag/FRT/V5-His-TOPO™) WebNov 8, 2013 · The Flp-In CHO cell line contains a single integrated FRT site and stably expresses the lacZ-Zeocin fusion gene. Flp-In CHO cells were cultured in Ham’s F12 medium (Nissui, Tokyo, Japan) containing 100 U/ml of penicillin (Meiji Seika, Tokyo, Japan), 100 μg/ml of streptomycin (Meiji Seika), 100 μg/ml of Zeocin (Invitrogen) and 10% fetal ... WebFlp-In™ 细胞系设计用于快速生成稳定细胞系以从 Flp-In™ 表达载体表达关注的蛋白。这些细胞在转录活性基因组基因座处包含一个稳定整合的 FRT 位点。Flp-In™ 表达载体的靶向 … green carpet cleaners lacey wa

Invitrogen™ Flp-In™-CHO Cell Line - Fisher Sci

Establishment of Flp-InTM CHO cell lines with double expression …

WebAug 20, 2024 · The Flp-In system integrates a vector into cell lines at a specific genomic locus, but has not been used for integration of more than one gene. ... X. et al. … WebJan 15, 2024 · We also established CHO cells expressing the WT protein using the Flp-In system as previously described (thereafter called Flp-In CHO-WT cells) [6]. The pcDNA5/FRT-PiT-2-flag in GFP vectors were obtained by inserting GFP into Loop7 of the SLC20A2 (WT, T115 M, and R467X) using in-fusion cloning system (Takara Bio Inc.; … flow inflating bag nrpWebJun 21, 2024 · Among different recombinases, the Flp/FRT system has been extensively applied in CHO cells to express monoclonal antibodies 35 and recombinant proteins 34, 36 probably due to its high... green carpet classic living room

"WebAll of the Flp-In ™ cell lines (except Flp-In -CHO; see the following section) contain a single integrated FRT site and stably express the lacZ-Zeocin™ fusion gene from the pFRT/lacZeo plasmid under the control of the SV40 early promoter (see the following diagram). The location of the FRT site in each " - Flp-in cho

Flp-in cho

Diagnostic Tests - Atlanta Heart Associates

WebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably … WebFlp-In™ T-REx™-293 3 × 106 cells, frozen R780-07 Flp-In™ Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™ host cell lines that stably express the lacZ-Zeocin™ fusion gene from pFRT/lacZeo or pFRT/lacZeo2 (Flp-In™-CHO). Each cell line contains a single integrated FRT site

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WebAug 8, 2016 · MIL75-pFRT-FTMK and POG44 were co-transfected (1:9) into Flp-In-CHO cells. After 48 h of transfection, the cells were cultured under selection pressure by hygromycin B (1 mg/ml). WebFRT site in the Flp-In™-CHO™ cell line has not been mapped, but has been demonstrated to have integrated into a highly transcriptionally active genomic locus as determined by …

WebDec 1, 2024 · Background: Many studies have investigated the characteristics and biological activities of type III interferon (IFN), finding that it has similar features to type I IFN but …

WebOct 1, 2024 · Objective To construct a double transfected Flp-InTM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and … WebThe Flp-In™ System allows for stable integration and expression of your gene of interest to deliver single-copy isogenic cell lines. Flp-In™ expression involves introduction of a Flp …

WebA single gene copy of shRNA-2 also decreased the translational level of DHFR by 80% in Flp-In CHO cells. shRNA-2 was then incorporated into a plasmid vector expressing human erythropoietin (EPO) and an exogenous DHFR to develop …

WebJul 17, 2024 · In order to test the Flp-in tm-ready cells, Lipofectamine 2000 was used to transfect 5 × 10 4 Lec 3.2.8.1 or 4 × 10 5 DG44 CHO cells with our GOI, in this case 100 ng of circular AQP3-GFP_pcDNA5/FRT … flow inflating bagWebOct 1, 2024 · Objective To construct a double transfected Flp-InTM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and multidrug resistance-associated protein 2(MRP2). flowinfoWebDec 1, 2024 · CHO ori cell line was isolated and immortalized by Theodore Puck in 1956 [7]. Subclones were then further developed by introducing mutations or via adaptation to … green carpet algae in fish tankWebDiagnostic Tests. ANKLE/BRACHIAL INDEX. BLOOD PRESSURE TEST. CARDIAC CATHETERIZATION. CARDIAC STRESS TESTING. CAROTID ULTRASOUND. CORONARY CALCIUM SCAN. CORONARY COMPUTED TOMOGRAPHY ANGIOGRAPHY (CCTA) ECHOCARDIOGRAPHY. flowinfo 1WebMar 5, 2024 · Idiopathic Basal Ganglia Calcification (IBGC) is a rare neuropsychiatric illness also known as Fahr's disease or Primary Familial Brain Calcification (PFBC). IBGC is caused by SLC20A2 variants, which encodes the inorganic phosphate (Pi) transporter PiT-2, a transmembrane protein associated with Pi homeostasis. green carpet cleaner bremerton waWebFeb 11, 2024 · Results: A range of sialyltransferase from mammals, birds and fish were stably expressed in CHO Flp-In cells. The stable cell lines expressing ST6Gal1 modify the glycans on the surface of the CHO cells as detected by fluorescently labelled lectin microscopy. The catalytic domains, as isolated by Ni Sepharose from culture media, … flowin fitness equipmentWebJan 21, 2011 · The transport assay for other sphingolipids was performed with Flp-In-CHO/SPHK1 cells stably expressing each transporter as described previously. The … flow infographic